By Wan Azlina Ahmad, Wan Yunus Wan Ahmad, Zainul Akmar Zakaria, Nur Zulaikha Yusof
Environmental issues concerning non-stop use of artificial dyes observed a revival within the call for for common dyes as typical dyes express larger biodegradability and usually have a better compatibility with the surroundings. in spite of the fact that, one of many obstacles at the use of ordinary dyes or pigments is the low extraction yield components (a few grams of pigment consistent with kg of dried uncooked material). hence, the exploitation of alternative organic resources resembling fungi, micro organism and mobilephone cultures deals attention-grabbing replacement. Microbial pigments similar to from bacterial origins supply the virtue when it comes to construction in comparison to pigments extracted from greens or animals, because of its uncomplicated mobile and quick culturing procedure. This ebook bargains attention-grabbing perception into preliminary works performed to illustrate the capability use of bacterial pigment as colorant for varied applications.
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Additional resources for Application of Bacterial Pigments as Colorant: The Malaysian Perspective
Upon pressing under 2,000 kPa, pellet disc obtained was analyzed using a FTIR spectrophotometer (FTIR 8300, Shimadzu) between 4,000 and 400 cm-1. The pigment (5–10 mg) was dissolved in 500 lL of chloroform-d2 (CDCl3) for NMR analysis (Min-jung et al. 2006). , 535 nm (Song et al. 2000). 40 2 Isolation of Pigment-Producing Bacteria Fig. 15 Different coloration of the red pigment at (a) pH 2 (b) pH 7 and (c) pH 9 Fig. 16 Maximum absorption of the red pigment at pH 2, 7 and 9; pigment dissolved in methanol The red-pigment changes to pink at pH 2, orange at pH 9 while retaining its red feature at pH 7 (Fig.
Violaceum was also recorded. Similar experimental setup as above minus the bacterial cells acted as a control. Growth of C. violaceum in NB was monitored for 24 h (Fig. 1). The bacteria showed a typical growth curve with distinct log, stationary and death phase. Lag phase was not observed as exponential growing culture was used to inoculate the medium causing cells to commence growth immediately. Optical density reading decreased slightly but increased and remained constant till 24 h. This might be due to the formation of precipitates after 5 h which might interfere with the optical density readings.
According to this method. According to Bernardet et al. 2002, since the color shift may pass unnoticed when the KOH solution is poured directly over a thin colony on an agar plate, it is strongly recommended that the test be performed on a small mass of bacterial cells collected with a loop and deposited on a glass slide placed on a white background. Another similar mass of bacteria on which no KOH is poured may be used as a control. The color changed induced by KOH is not absolutely specific for the flexirubin-type of pigment (Fautz and Reichenbach 1980), but it is still helpful when combined with the results of other tests.
Application of Bacterial Pigments as Colorant: The Malaysian Perspective by Wan Azlina Ahmad, Wan Yunus Wan Ahmad, Zainul Akmar Zakaria, Nur Zulaikha Yusof