Download PDF by Margaret A. Scofield, Jean D. Deupree, David B. Bylund: Adrenergic Receptor Protocols

By Margaret A. Scofield, Jean D. Deupree, David B. Bylund (auth.), Curtis A. Machida (eds.)

ISBN-10: 0896036022

ISBN-13: 9780896036024

In Adrenegic Receptor Protocols, Curtis Machida and a panel of specialist investigators current a entire choice of glossy molecular equipment for interpreting adrenergic receptors and corresponding moment messenger structures. those confirmed and effectively reproducible recommendations make the most of genetic, RNA, protein expression, transactivator, and moment messenger methodologies, in addition to immunocytochemical, electrophysiological, transgenic, and in situ hybridization ways. all of the specialists writing right here information using their selected technique in studying the adrenergic receptor process, utilizing points of the genetic circulation of knowledge as a advisor: DNA ' RNA ' transactivator ' protein expression ' moment messenger analyses ' mobile analyses ' transgenic entire animal approaches.
entire and wealthy in functional element, Adrenergic Receptor Protocols offers the 1st number of reproducible tools for the examine of those vital regulators of CNS-mediated habit and neural functionality. Its cutting-edge tools represent trendy optimum reference for all neurobiologists, neurochemists, neurologists, and pharmacologists learning this exceedingly very important classification of receptors.

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Yu, L. and Bloem, J. J. (1996) Use of polymerase chain reaction to screen phage libraries in Methods in Molecular Biology, vol. 58: Basic DNA and RNA Protocols, (Harwood, A. ), Humana, Totowa, NJ, pp. 335–339. 3. Takumi, T. and Lodish, H. F. (1994) Rapid cDNA cloning by PCR screening. Biotechniques 17, 443–444. 4. , Kingston, R. , Moore, D. , Seidman, J. , Smith, J. , et al. (1995) Mapping by multiple endonuclease digestions, in Current Protocols in Molecular Biology, vol. 1. Wiley, Interscience, New York.

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Sennitt, M. , Thody, V. , et al. (1984) Atypical β-adrenoceptors on brown adipocytes as a target for anti-obesity drugs. Nature 309, 163–165. Bond, R. A. and Clarke, D. E. (1988) Agonist and antagonist characterization of a putative adrenoceptor with distinct pharmacological properties from the alphaand beta-subtypes. Br. J. Pharmacol. 95, 723–734. , Montastruc, J. , and Berlan, M. (1997) Lipolytic effects of conventional β3-adrenoceptor agonists and of CGP 12,177 in rat and human fat cells: preliminary pharmacological evidence for a putative β4-adrenoceptor.

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Adrenergic Receptor Protocols by Margaret A. Scofield, Jean D. Deupree, David B. Bylund (auth.), Curtis A. Machida (eds.)


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